Glucose (GLUC), Urine

CPT Code:





10 mL aliquot

Minimum Volume:

0.5 mL aliquot


Plastic urine container with boric acid or sodium fluoride



Patient Preparation:

For 24-hour:  Void at 8 AM (or 8 PM) and discard the specimen. Then collect all the urine including the final specimen voided at the end of the 24-hour collection period (ie, 8 AM (or 8 PM) the following day).


For 24-hour: Collect 24-hour urine with 1 g boric acid or sodium fluoride preservative.

Mix well. Container must be labeled with patient's name and date and time collection was started and finished.

Reference Range:



Temperatures Period (not specified)


Glucose is the primary source of energy for all bodily functions. The blood glucose level is strictly regulated by several hormones, including insulin and glucagon.  Urine glucose measurements are used to aid in the evaluation of glucosuria, renal tubular defects; manage diabetes mellitus.

Clinical Significance:


High glucose is an indication of hyperglycemia. Other causes include nonfasting specimen; recent or current IV infusions of glucose; stress states such as myocardial infarct, brain damage, CVA, convulsive episodes, trauma, general anesthesia; Cushing disease; acromegaly; pheochromocytoma; glucagonoma; severe liver disease; pancreatitis; drugs (thiazide and other diuretics, corticoids, many others are reported to affect glucose). 
Low glucose is an indication of hypoglycemia.  Other causes include excess insulin, including rare insulin autoimmune hypoglycemia, surreptitious insulin injection, and sulfonylurea use.

Causes for Rejection:

Gross hemolysis; patient not fasting; blood stored overnight on clot; improper labeling

Interfering Substances:

Hemoglobin (hemolysate) at 1000 mg/dL decreases a GLUC result at 50 mg/dL by 11%. Bilirubin (unconjugated) at 60 mg/dL increases a GLUC result at 50 mg/dL by 13%. Lipemia at 200 mg/dL increase a GLUC result at 50 mg/dL by 10%. Pralidoxime iodide (PAM) concentration of 512 µg/mL increases a GLUC result of 78 mg/dL by 17%.

Pralidoxime iodide (PAM) concentration of 1024 µg/mL increases a GLUC result of 204 mg/dL by 13%.