Hemoglobin: CPT 85018

CPT Code: 



Whole blood 


Tube fill capacity 

Minimum Volume: 

0.5 mL 


Lavender-top (EDTA) tube


Invert tube 8 to 10 times immediately after tube is filled at the time of collection.

Storage Instructions:

 Maintain specimen at room temperature.


Temperature Period 
Room temperature 1 day 
Refrigerated 3 days 
Frozen  Unstable 
Freeze/thaw cycles Unstable


Causes for Rejection:   Hemolysis; clotted specimen; tube not filled with minimum volume; improper labeling; transport tubes with whole blood; specimen diluted or contaminated with IV fluid; specimen drawn in any anticoagulant other than EDTA 
Use:  Evaluate anemia, blood loss, hydration or suspected polycythemic condition, and response to treatment 
Limitations:  Hyperlipemic plasma (especially Fredrickson and Lees type I and V in which chylomicronemia is present) or white count >50,000/mm3 may falsely elevate the hemoglobin result with corresponding increase in the MCH. The laboratory will attempt to correct this interference by a special technique. 
Methodology:  Automated cell counter